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. 2014 Aug 11;3(8):e113. doi: 10.1038/oncsis.2014.26

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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miRNA-resistant cyclin D1 reverses the miR-206-induced decrease in cell survival. MDA-MB-236 cells were co-transfected with a CCND1 expression vector or empty vector control and miR-ve or miR-206. Twenty-four hours later, cells were (a) harvested for western blot analysis. (b) Analyzed for clonogenic survival. n=3 **P=0.019. (c) Graphical representation of three independent experiments. Values are mean±s.e.m. Percentage cell survival was determined with control miR-ve set at 100%. A paired t-test was used to determine the significance between miR-ve and miR-206 expressing cells, **P=0.006.