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Prostatic cancer cells lack a CCND1 3′-UTR. RNA from PrEC, DU145, LNCaP, PC-3 and MDA-MB-231 cells was reversed transcribed into cDNA. qPCR was used to amplify (a) the CCND1 ORF and (b) the CCND1 3′-UTR from these various cell lines. Shown is the average relative quantity (±s.e.m.) of four experiments normalized to TATA-binding protein (TBP) and to the amount of RNA expressed in PrEC.
