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. 2016 Apr 26;7(28):43124–43149. doi: 10.18632/oncotarget.8988

Figure 9. Effect of sulindac sulfide, WntC59, XAV939, GDC-0941 treatment and transient transfection of beta-catenin siRNA on real-time proliferation (A), fibronectin-directed migration (B) and clonogenic 3D growth (C) of brain metastasis-directed MDA-MB231BR TNBC cells.

Figure 9

MDA-MB231BR cells were used to test the role of the WP directly in real time proliferation A. fibronectin-directed migration B. and 3D clonogenic growth C. under different study conditions e.g. physiological stimulation of the WP by LWnt3ACM and genetic perturbation of beta-catenin following transient transfection of beta-catenin siRNA. (A) Sulindac sulfide blocked LWnt3ACM stimulated proliferation of cells as measured by the percentage of the confluence of cells (mean Vs time). (B) LWnt3ACM stimulated fibronectin-directed migration for 24 hours was abrogated in the presence of WP modulators, WntC59 10 nM as well as XAV939 (5 μM), sulindac sulfide (50 μM) and GDC-0941 (1 μM). C. LWnt3ACM stimulated 3D clonogenic growth for 48 hours was abrogated following transient transfection of beta-catenin siRNA as compared to the transfection of scrambled siRNA.