Skip to main content
. 2016 Jun 1;7(28):43199–43219. doi: 10.18632/oncotarget.9774

Figure 1. NW457 reveals no detectable hepatocytotoxicity in vitro.

Figure 1

Primary hepatocytes of C57BL/6 mice were exposed to 0-300 nM of NW457, geldanamycin (GA), or DMSO as vehicle control. Cellular morphology and viability were assessed by immunofluorescence microscopy and Alamar Blue assays. A. Immunofluorescence microscopy was performed after 24 h. Cells were fixed, permeabilized, and stained with anti-tubulin-FITC, phalloidin-Alexa Fluor 568 for actin visualization, and Hoechst 33342 for DNA visualization. Scale bars represent 20 μm. B. Alamar Blue viability tests were performed after 24 and 48 h, and the results were calibrated on the vehicle controls (100% viability). Means ± s.d. of intra-assay quadruplicates of one representative of three experiments are depicted.