Figure 1. Pim kinases enhance endogenous Notch activity.

A. Endogenous expression levels for intracellular domains of Notch1 (N1ICD) or Notch3 (N3ICD), and for Pim family kinases were analysed by Western blotting from MCF-7 and PC-3 cell lysates. β-actin levels were used as loading controls. B. Endogenous Notch activity was measured by CSL-dependent luciferase reporter assays in MCF-7 transiently transfected with non-targeting (NT) or Pim gene-specific siRNAs. RLU, relative light unit. C. Similar assays were carried out in cells transiently transfected with an empty control vector (C), wild-type (WT) or kinase-deficient (KD) Pim1. D-G. CSL- or CMV-dependent luciferase reporter assays were performed with untransfected MCF-7 or PC-3 cells that had been treated for 24 h with 0.1% DMSO (−), 10 μM DHPCC-9 or 10 μM SGI-1776. Shown are representative graphs from three independent luciferase experiments with average data from three parallel samples.