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A. To visualize localization of endogenously expressed proteins, MCF-7 cell samples were stained with antibodies that recognized either Pim1 or both full-length and cleaved Notch1. B. In situ proximity ligation assay (PLA) was used to demonstrate physical interaction between Pim1 and Notch1. Notch1 alone or in combination with cytochrome oxidase II (CoxII) were used as negative controls.
