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. 2016 May 7;7(28):43220–43238. doi: 10.18632/oncotarget.9215

Figure 7. Phosphorylation of Notch1 increases PC-3 cell migration.

Figure 7

A. PC-3 cell migration was analysed by wound healing assays in cells transiently transfected with Pim1 and/or treated with 0.1% DMSO, 10 μM DHPCC-9 or 5 μg/ml of DAPT for 24 h. Pim expression levels from the wound healing samples were analysed by Western blotting. B. Similar wound healing assays were performed with PC-3 cells that had been plated onto wells coated with the Notch ligand Jagged1. C. N1ΔE wild-type (WT), phosphodeficient (SA) or phosphomimicking (SE) mutants were overexpressed in PC-3 cells, in which cell migration was analysed after treatments with 0.1% DMSO or 10 μM DHPCC-9. Shown are average healing percentages from two or three independent experiments.