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. 2016 May 7;7(28):43220–43238. doi: 10.18632/oncotarget.9215

Figure 8. Phosphorylation of Notch1 balances MCF-7 cell metabolism.

Figure 8

A. Untransfected MCF-7 cells were treated overnight with 0.1% DMSO or 10 μM DHPCC-9, after which glucose uptake was measured by flow cytometry, using the fluorescent 2-NBDG probe. B. Mitochondrial membrane potential was similarly measured by flow cytometry, using the TMRM probe. C. Glucose uptake was also measured from cells transiently transfected with wild-type or mutant N1ΔE. D. Lactate production was measured after similar transfections and/or DHPCC-9 treatment. E. Lactate production was also measured after Pim1 transfection and/or DHPCC-9 treatment. Shown are average values from two to three independent experiments.