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. 2016 May 27;7(28):43256–43266. doi: 10.18632/oncotarget.9673

Figure 6. Depletion of Xist or SPEN increases HDAC3 recruitment to the PHLPP1 promoter and decreases PHLPP1 expression.

Figure 6

(A and B) M10 cells were transfected with 10 nM specific siRNA oligonucleotides against Xist or SPEN using RNAiMAX then subjected to chromatin immunoprecipitation (ChIP). ChIP analyses were performed with antibodies against HDAC3, acetylated histone H3 (H3-ac) or an IgG control. Subsequent qPCR analysis was carried out by primers specific for PHLPP1 promoter. Input represents 1% of the chromatin used for immunoprecipitation. (C and D) M10 cells were transfected with Xist siRNA and/or SPEN siRNA using RNAiMAX then subjected to quantitative RT-PCR (C) and Western analyses (D). Error bars: mean ± SD, n = 3.