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. 2016 May 30;7(28):43419–43431. doi: 10.18632/oncotarget.9728

Figure 2. Overexpression of miR-1258 inhibits liver cancer cell growth and proliferation.

Figure 2

(A) The normalized relative expression of miR-1258 in a panel of liver cancer cells and two normal liver tissues by RT-qPCR analysis. (B) The expression of miR- 1258 was significantly increased in HuH7 and HCCLM3 cells transfected with p-miR-1258 plasmid compare with p-miR-control plasmid transfection or normal control (NC). (C and D) Overexpression of miR-1258 in HuH7 (C) and HCCLM3 (D) significantly inhibits liver cancer cells growth by MTS analysis. (E) The represent images of soft agar colony formation in HuH7 and HCCLM3 cell transfected with p-miR-1258 or p-miR-control. (F) The number of colonies formed in in HuH7 and HCCLM3 cell transfected with p-miR-1258 or p-miR- control was counted under the microscope. All data are given as mean ± SD of three independent experiments. Significant differences are indicated, *P < 0.05.