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. 2016 May 30;7(28):43419–43431. doi: 10.18632/oncotarget.9728

Figure 3. miR-1258 induces cell cycle arrest in G0/G1 phase and promotes cell apoptosis.

Figure 3

(A) Cell cycle distribution and apoptosis were evaluated by flow cytometry analysis performed 2 days after HuH7 and HCCLM3 cell transfected with p-miR-1258 or p-miR-control. Note that overexpression of miR-1258 induced cell cycle arrest in G0-G1 phase and dramatic increase in the percentage of cells with sub-G0 DNA content (indicative of apoptosis). (B) Representative images of Annexin V/7-AAD apoptotic assay showing that the percentage of Annexin V+ apoptotic cells was significantly increased in HuH7 and HCCLM3 cell transfected with p-miR-1258 in comparison to the p-miR-control transfected cells.