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. 2016 May 30;7(28):43419–43431. doi: 10.18632/oncotarget.9728

Figure 5. CKS1B was identified as the critical downstream target of miR-1258.

Figure 5

(A) The binding site of CKS1B with miR- 1258 was predicted by TargetScanHuman 7.0 (A) or miRDB (B) (http://mirdb.org/miRDB/). (C) The normalized relative luciferase activity of HCCLM3 cells contransfected with p-miR-1258 or p-miR-control and pGL3- CKS1B-3′UTR wide type or pGL3- CKS1B-3′UTR-mutatant. (D) The representative images of western blot analysis showing that overexpression of miR-1258 inhibits the expression of CKS1B. The downstream p27 was increased while IL8 was decreased. (E) The normalized relative expression of CKS1B in 20 pairs of HCC and adjacent normal samples by RT-qPCR analysis. (F) The significant correlation between the expression of mR-1258 and CKS1B was observed in our tissue samples.