Figure 6. Re-expression of CKS1B overcomes miR-1258 induced apoptosis and increases stemness.

(A) The representative images of western blot analysis showing that re-expression of CKS1B inhibits p27 expression and promotes IL8 expression. (B) Re-expression of CKS1B significantly promotes liver cancer cells growth by MTS analysis. (C) The represent images of soft agar colony formation showing that re-expression of CKS1B promotes liver cancer cell growth. (D) Representative images of Annexin V/7-AAD apoptotic assay showing that the percentage of Annexin V+ apoptotic cells was significantly decreased by CKS1B overexpression. (E) The represent images of sphere formation showing that re-expression of CKS1B promotes liver cancer cell sphere formation ability.