Figure 7. Stable overexpression of miR-1258 decreases tumorigenicity of HCC cells.

(A) Determination of the tumor growth. Tumor volume was calculated every week after injection. Data were mean ± SD of three independent experiments. *P = 0.0012. (B) Representative image of tumor formation. Nude mice were subcutaneously injected with 5 × 10⁶ P-miR-1258 or P-miR-control stable transfected HCCLM3 cells. (C) The Representative images of immunohistochemistry (IHC) analysis showing that the expression of CKS1B was significant decreased while p27 was significantly increased by overexpression of miR-1258. (D) Representative images of TUNEL staining of tumor tissues showing that overexpression of miR-1258 significantly promotes tumor cell apoptosis.