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. 2016 Jun 2;7(28):43570–43587. doi: 10.18632/oncotarget.9781

Figure 3. Extracellular vesicles secreted from HiMet cells increase in vitro migration and invasion of LoMet cells.

Figure 3

EVs were prepared from HiMet-C6 or LoMet-C4 clones and incubated with LoMet-C4 or C5 clones for 24 hr. (A) Increase in the migration of LoMet-C4 and C5 in the presence of EVs secreted by a HiMet-C6. *1-way ANOVA (F = 3.97–4.13, P = 0.032–0.04), Tukey's MCT for P < 0.05. (B) Increase in the invasion of LoMet-C4 and C5 in the presence of EVs secreted by HiMet-C6. *1-way ANOVA (F = 4.15, P = 0.036), Tukey's MCT for P < 0.05. (C) Increase in migration of LoMet-C4 cells in the presence of HiMet-C6 EVs is not due to the proliferation of LoMet-C4 cells, measured as DNA synthesis (BrdU incorporation). 1-way ANOVA with Tukey's MCT = ns. (D) LoMet-C4-secreted EVs do not contribute to the increase in migration of LoMet-C4 cells. 1-way ANOVA with Tukey's MCT = ns. Data presented as mean +/− SD from triplicate determinations of at least 2 experiments. Bars: SD.