Figure 5. Expression of PD-1 and PD-L1 on CIK cells within large-scale culture system was detected.
PBMCs from ten individuals with NSCLC were chosen for large-scale CIK culture. (A–C) During 15 days of culture, expression of PD-1 and PD-L1 on CD3+ CD4+ T, CD3+CD8+ T and CD3+CD56+ NKT cells was detected at day 0, 3 and 15 by FCM. Data represent the mean ± SD of ten independent experiments. (D–F) Expression of TIM-3 and CEACAM1 on CD3+ CD4+ T, CD3+ CD8+ T and CD3+ CD56+ NKT cells was detected at day 0, 3 and 15 by FCM. Data represent the mean ± SD of ten independent experiments.