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. 2016 May 27;7(28):43654–43668. doi: 10.18632/oncotarget.9661

Figure 8. TRPML-2 does not influence autophagic pathway.

Figure 8

A. Lysates from U251 untransfected, siGLO and siTRPML-2, treated or not with rapamycin (50 nM) for 72 h, were separated on 14% SDS-PAGE and probed with anti-LC3, p62 and anti-GAPDH Abs. GAPDH protein levels were evaluated as loading control. Blots are representative of one of three separate experiments. *p<0.01 vs U251; #p<0.01 vs U251 siTRPML-2;§p<0.01 vs U251 siGLO or U251 siTRPML-2 (Anova with Bonferroni's post-test). B. Lysates from T98 untransfected, siGLO and siTRPML-2, treated or not with rapamycin as above described, were separated on 14% SDS-PAGE and probed with anti-LC3, p62 and anti-GAPDH Abs. GAPDH protein levels were evaluated as loading control. Blots are representative of one of three separate experiments. *p<0.01 vs T98; #p<0.01 vs T98 siGLO or T98 siTRPML-2 (Anova with Bonferroni's post-test).