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. 2016 May 27;7(28):43779–43791. doi: 10.18632/oncotarget.9672

Figure 4. AKR1B10 activates the focal adhesion-Rac1 signaling cascade.

Figure 4

A. Activation of focal adhesion signaling cascade, showing p-FAK397, p-FAK925, p-Src and p-paxillin levels in indicated cells. Scram, scrambled siRNA; siR-1, AKR1B10 siRNA-1; siR-2, AKR1B10 siRNA-2. B. Rac1-mediated cell migration. Upper left: Active Rac1 (a-Rac1) examined by pull-down assays with PAK-PBD beads. Active Rac1 pulled down and total Rac1 (t-Rac1) in cell extracts were assessed by Western blot. Lower panel images: Effects of Rac1 inhibitor, EHop-016, on MCF-7 cell Transwell migration. Upper right: Quantitation of migrated cells. Data denote mean ± SD from three independent experiments. ** p<0.01, compared to EHop-016-treated MCF-7 cells with AKR1B10 expression or with a vector control. ## p<0.01 compared to EHop-016-treated MCF-7 cells with AKR1B10 expression or with a vector control.