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. 2016 May 30;7(28):43835–43851. doi: 10.18632/oncotarget.9699

Figure 3. LncRNA RP11-838N2.4 enhances sensitizes of TMZ in U87TR and U251TR.

Figure 3

A. Fluorescent in situ hybridization of the lncRNA RP11-838N2.4 in U87 and U251 cells. DAPI was represented the nuclear and lncRNA 18s probe was used as cytoplasm control. Scale bar: 20μm. B, C. the level of lncRNA RP-11-838N2.4 was screened by qRT-PCR in U87TR (B) and U251TR (C) cells that transfected with pcDNA-RP or pcDNA-NC. D, E. U87TR and U251TR cells were transfected with pcDNA-RP or pcDNA-NC. The viability was measured by CCK-8 (D) and the sensitivities to TMZ were calculated (E) after GBM cells transfected with indicated constructs. F, G. U87TR and U251TR cells were transfected with pcDNA-RP or pcDNA-NC, then cultured with 0μg/ml or 50μg/ml TMZ for 48h. The rate of apoptosis cells was measured by FACS-based Annexin-V/FITC double staining (F) and TUNLE (G). The bar graph shows the percentage of apoptotic cells. (G) Cell nuclear stained with DAPI, and apoptotic cell stained with TUNEL. The TUNEL positive cells were counted in eight randomly selected fields at 200 × magnification. TMZ, temozolomide. Data are represented as mean±SD of three independent experiments.*P < 0.05, **P < 0.01.