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. 2016 May 30;7(28):43835–43851. doi: 10.18632/oncotarget.9699

Figure 6. LncRNA RP11-838N2.4 reverses the inhibitory effect of miR-10a on TMZ sensitivity of GBM.

Figure 6

A-D. U87TR and U251TR cells were transfected with anti-miR-10a or anti-miR-control. U87 and U251 cells were transfected with miR-10a or miR-control. The viability was measured by CCK-8 (A, B) and the sensitivities to TMZ were calculated (C, D) after GBM cells transfected with indicated constructs. E-J. U87TR and U251TR cells were transfected with miR-10a+pcDNA-NC, miR-10a+pcDNA-RP (wild) or miR-10a+pcDNA-RP (mut). The viability was measured by CCK-8 (E, F) and the sensitivities to TMZ were calculated (G, H) after GBM cells transfected with indicated constructs. Cultured with 0μg/ml or 50μg/ml TMZ for 48h, GBM cells apoptosis rate was measured by FACS-based Annexin-V/FITC double staining (I) and TUNEL (J). The TUNEL positive cells were counted in eight randomly selected fields at 200 × magnification. The bar graph shows the percentage of apoptotic cells. TMZ, temozolomide. *P < 0.05, **P < 0.01.