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. 2016 Jun 7;7(28):44129–44141. doi: 10.18632/oncotarget.9866

Figure 5. Silencing DNMT1 up-regulated expression of RASSF1A and DAPK.

Figure 5

A. The mRNA expression of DAPK, MGMT, RASSF1A, APC, DNMT1, ASC, P16 and CDH13 in ESCC stable cells. mRNA expression of tumor suppressors was normalized to GAPDH. Data represented as means ± SD. *, p< 0.05. **, p < 0.01. B. The protein expression of DAPK and RASSF1A in ESCC cells. GAPDH was served as loading control. C. The mRNA expression of DAPK, RASSF1A and DNMT1 in tumors isolated from nude mice injected with ESCC stable cells. mRNA expression of tumor suppressors was normalized to GAPDH. Data represented as means ± SD. *, p < 0.05. **, p < 0.01. D. The protein expression of DAPK and RASSF1A in tumors isolated from nude mice injected with ESCC stable cells. GAPDH was served as loading control. Data represented as means ± SD. *, p< 0.05. **, p< 0.01. E. IHC staining of DAPK and RASSF1A in tumors isolated from nude mice injected with ESCC stable cells.