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. 2016 Jun 13;7(28):44211–44223. doi: 10.18632/oncotarget.9954

Figure 3. Deletion of PARK2 induced chromosome instability.

Figure 3

A. PARK2 deficiency results in centrosome amplification in MEFs at passage 5. (left) Immunofluorescence staining shows impaired mitoses in MEFs depleted of PARK2. Red, γ-tubulin; White arrows, centrosome in mitosis. The scale bar is 20 μm. (right) Quantification of 2 < centrosomes cells. ***, p < 0.001 by one-way ANOVA. n=10. B. After infection with the indicated plasmids, MEFs lysates were fixed for immunofluorescence assay. Quantification of 2 < centrosomes cells using γ-tubulin. Results are shown as means (±SEM), and at least three experiments were performed for all experiments. *, p < 0.05 and **, p < 0.01 by one-way ANOVA. C. Immunofluorescence staining shows impaired mitoses in IMR 90 lung fibroblast cells depleted of PARK2. White arrows, centrosome in mitosis. Yellow arrows, abnormal chromosome. The scale bar is 20 μm. D. Cell were harvested at indicated times and analyzed by immunobloting. E. Impaired mitoses including multipolar spindles, misalignment and abnormal microtubule in PARK2 knockout (KO) MEF cells. Yellow arrows, abnormal chromosome. The scale bar is 20 μm. Results are shown as means (±SEM), and at least three experiments were performed for all experiments. **, p < 0.01 by one-way ANOVA.