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. 2016 Jun 13;7(28):44211–44223. doi: 10.18632/oncotarget.9954

Figure 4. PARK2 loss leads to tumor initiation and overexpression of PARK2 inhibits cell proliferation in lung cancer cell lines.

Figure 4

A. Primary MEFs were applied on 3-D cultures system for 2 weeks and counted the sphere of the cells. (top) Representative images indicate the sphere cells. (bottom) Quantification of the sphere number. ****, p < 0.0001 by one-way ANOVA. n=6. (B. and C.) PARK2 WT and KO MEFs were analyzed for foci formation at passage 5 and 21 (B), and colony formation at passage 21 C. The scale bar is 20 μm. Results are shown as means (±SEM), and at least three experiments were performed for all experiments. NAC, ROS scavenger. ***, p < 0.001 by one-way ANOVA. D. The growth of lung cancer cell lines (H1437, H522, H1650, A549, H460, H1299 and H196) was inhibited through infection with PARK2. Cells were transfected with the indicated plasmids, and then cell lysates were blotted with the indicated antibodies. E. The percentage of lung cancer cell growth compared to controls after transfected PARK2. Results shown as means (± SEM), and at least three experiments were performed for all experiments. **, p < 0.01 and ***, p < 0.001 by one-way ANOVA. F. PARK2 and β–actin expression was measured by immunoblot.