Figure 3. TERF2 down-regulation does not alter proliferation and invasion of CAL33 cells. A. Expression of TERF2 was tested in human keratinocytes (HK), CAL33 cells expressing scramble (shC) or two independent shRNA directed against TRF2 (sh1, sh2). Tubulin is shown as a loading control. B. Quantification of the blot shown in A: ***P < 0.001. C. Cumulative population doublings (CPD) of shC, sh1 and sh2 CAL33 cells. D. Clonal growth of shC, sh1 and sh2 CAL33 cells. E. Invasive properties of shC, sh1, sh2 CAL33 cells in matrigel. Quantification of the spheroid area of three independent experiments is shown.