Figure 5. TERF2 down-regulation destabilized tumor vessels and induced fibrosis. A, F. Immunohistochemical staining for TERF2. B, G. General histological aspect of shC or sh2 tumors stained with trichrome. The black arrowhead shows collagen surrounding the vessels. Mean collagen thickness around vessels was measured in shC and sh2 tumor sections. C, H. Trichrome staining also shows immune cell extravasation (white arrowhead) and blood cells extravasation (red arrowhead). D, I. Tumor vasculature was detected with CD31 (endothelial cells, green) and α-SMA immuno-staining (pericytes, red) and nuclei were labeled with DAPI (blue). Higher magnification showing pericyte coverage of blood vessels in shC tumors E. and diffuse pericytes in sh2 tumors J.
