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. 2016 Jun 14;7(28):44266–44276. doi: 10.18632/oncotarget.10023

Figure 4. CD276 is a direct target of miR-187.

Figure 4

A. Expression level of CD276 and Dab2 in CRC cell lines and the normal NCM460 cells. B. The mRNA level of CD276 and Dab2 were detected in the presence of miR-187 mimics or miR-187 inhibitor (**P < 0.01, ***P < 0.001). C. The protein level of CD276 in SW620 and HT29 cells was detected in the presence of miR-187 mimics by Western blotting. D. The predicted sites of miR-187 binding to the 3′-UTR region of CD276 were detected using bioinformatics prediction tools. The mutated site in the 3′-UTR region of CD276 was also shown. E. SW620 cells were co-transfected with miR-187 expression vector or the empty vector and CD276 3′-UTR reporter plasmid or its mutant form. And the luciferase activity was detected 48 h after transfection. The data are shown as the means ± S.D. of three replicates (**P < 0.01).