Figure 3. HMGA2 sustains GIC self-renewal and tumorigenicity.
A. MTT assay showing effects of HMGA2 knockdown on GIC cell propagation in adherent cultures. B. Representative images showing TPC1115 and TPC0411 GICs maintained in neurosphere conditions for 7 days after transducing with indicated lentiviruses. C. Quantification of sphere numbers and diameters of three independent experiments in (B). D. Quantification of Ki67- (left) and BrdU- (right) labeled TPC1115 GICs and U251 glioma cells upon depletion of HMGA2. E. Xenografted nude mice were perfused with 4% PFA 10 weeks after intracranial TPC1115 transplantation (1×105) and brains were dissected out. Fluorescent images of brains were captured using the Maestro In-vivo Imaging System. Scrb, scramble shRNA; sh#(1-2), shHMGA2#(1-2). Scale bar: 1mm.