Figure 1. Piezo2 knockdown suppresses tumor growth.
A-B. An in vivo glioma model was established by injection of GL261 cells pre-transfected with scrambled shRNA (negative control, NC) or Piezo2 shRNA. Tumor volume was daily detected using a caliper and calculated by the formula: 0.52 × [width]2 × [length] (A, n=6 animals per group). The tumors were weighed immediately after isolation from mice. Tumor weight was plotted between the two groups (B, n=6 animals per group). Ki67 staining was conducted to detect cell proliferation. A representative image and statistical result was shown. Scar bar: 20 μm (C, n=6 animals per group). TUNEL was counterstained with DAPI (blue) for nuclei labeling. Scar bar: 20 μm. The number of Ki67-positive or TUNEL-positive cells was normalized to total nuclei number. * P<0.05, Piezo2 shRNA group versus NC group (D, n=6 animals per group). All data were from three independent experiments.