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. 2016 Jun 17;7(28):44630–44643. doi: 10.18632/oncotarget.10134

Figure 2. Piezo2 is localized in tumor endothelial cells, and regulates tumor angiogenesis and vascular leakage.

Figure 2

A. An in vivo glioma model was established by injection of GL261 cells pre-transfected with scrambled shRNA (negative control, NC) or Piezo2 shRNA. Tumors were excised on day 14 after implantation. Sections were double-labeled for Piezo2 (green) and vascular endothelium (CD31, red), and counterstained with DAPI (blue; n=6 animals per group). Scar bar: 20 μm. B-D. Excised tumors were also stained using CD31 (red) and fibrinogen (green) antibody, and then counterstained with DAPI (blue). Scar bar: 20 μm. To quantify angiogenic areas in tumor tissue, CD31 staining was quantified relative to the total pixel density (C, n=6 animals per group). Fibrinogen deposition was normalized to total CD31-positive pixel density (D, n=6 animals per group). The data was shown as relative change compared with NC group. E. Evans blue (30 mg/kg) was injected through the tail vein and circulated for 30 min. Tumors were excised and Evans blue concentration was quantified (n=6 animals per group). *P<0.05, Piezo2 shRNA group versus NC group. All data were from three independent experiments.