Figure 3.

Comparative responsiveness of Arabidopsis native NADK to various CML proteins. Activity of purified native NADK was examined in the presence of 1 mm EGTA or 2 mm Ca²⁺ and 100 nm of either the conserved CaM (CaM81) or 8 different Arabidopsis CMLs: CML10 (AAD12002), CML47 (CAB61974), CML3 (AAF02168), CML18 (AAF26959), CML42 (CAB79078), CML39 (AAF04446), CML2 (CAB78328), and CML43 (BAB09153). The CMLs were cloned, expressed as recombinant proteins in E. coli, and purified as described in “Materials and Methods.” Data represent mean ± se from triplicate assays of a typical experiment. A sequence alignment of various CML proteins can be found as supplemental data (Supplemental Fig. 2).