Figure 8.

Gene expression analysis of StSXD1-silenced plants. Total RNA was isolated from lower and upper source leaves (11th and 8th leaves from the top of 59-d-old StSXD1-RNAi lines and control plants (wild type), and transcript levels were analyzed by northern blotting. Thirty micrograms of RNA were loaded per lane and hybridized with cDNA probes of photosynthetic (rbcS, cab), defense-related (Pin2), as well as JA (AOC) and Pro (P5CS) biosynthetic genes. Additional probing with an 18S cDNA served as loading control.