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. 2004 Jul;135(3):1280–1293. doi: 10.1104/pp.104.041970

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Copyright © 2004, American Society of Plant Biologists

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Figure 1. — Characterization of NtCaMK1 cDNA. A, Nucleotide and deduced amino acid sequences of NtCaMK1 (AF550608) from tobacco. Nucleotides and amino acid residues are numbered on the right. Start (ATG) and stop (TAG) codons are boxed. The stop codon (TGA) in frame in the 5′ untranslated region is indicated by gray squares at positions 61 to 63. The putative CaM-binding domain is underlined. The strong PEST sequences are in bold [452–466 (+8.75), 481–506 (+9.02), 1241–1272 (+8.63)]. The 33 amino acid repeats are double underlined. B, Alignment of NtCaMK1 with other kinases, including CaM-binding protein kinases and CDPKs. The kinases are: maize MCK1 (accession no. 1839597; Lu et al., 1996), rat brain CaMKII α-subunit (accession no. J02942; Lin et al., 1987), tobacco NtCBK2 (accession no. AF435452; Hua et al., 2003), maize MCKI (accession no. 1839597; Lu et al., 1996), Arabidopsis CRK5 (accession no. At3g50530), lily CCaMK (accession no. 860675; Patil et al., 1995), soybean CDPK (accession no. P28583; Harper et al., 1991), and Arabidopsis CDPK3 (accession no. 1220099; Urao et al., 1994). Identities between NtCaMK1 and other kinases are indicated by gray squares. C, Diagram showing genomic structure of NtCaMK1. The introns are shown by boxes and exons with lines. The numbers above the boxes indicate the size of the introns (in bp) and the numbers above the lines indicate the size of the exons (in bp). A larger version of this figure is available at http://www.plantphysiol.org/cgi/content/full/135/3/1280/DC1.

Figure 1. — Characterization of NtCaMK1 cDNA. A, Nucleotide and deduced amino acid sequences of NtCaMK1 (AF550608) from tobacco. Nucleotides and amino acid residues are numbered on the right. Start (ATG) and stop (TAG) codons are boxed. The stop codon (TGA) in frame in the 5′ untranslated region is indicated by gray squares at positions 61 to 63. The putative CaM-binding domain is underlined. The strong PEST sequences are in bold [452–466 (+8.75), 481–506 (+9.02), 1241–1272 (+8.63)]. The 33 amino acid repeats are double underlined. B, Alignment of NtCaMK1 with other kinases, including CaM-binding protein kinases and CDPKs. The kinases are: maize MCK1 (accession no. 1839597; Lu et al., 1996), rat brain CaMKII α-subunit (accession no. J02942; Lin et al., 1987), tobacco NtCBK2 (accession no. AF435452; Hua et al., 2003), maize MCKI (accession no. 1839597; Lu et al., 1996), Arabidopsis CRK5 (accession no. At3g50530), lily CCaMK (accession no. 860675; Patil et al., 1995), soybean CDPK (accession no. P28583; Harper et al., 1991), and Arabidopsis CDPK3 (accession no. 1220099; Urao et al., 1994). Identities between NtCaMK1 and other kinases are indicated by gray squares. C, Diagram showing genomic structure of NtCaMK1. The introns are shown by boxes and exons with lines. The numbers above the boxes indicate the size of the introns (in bp) and the numbers above the lines indicate the size of the exons (in bp). A larger version of this figure is available at http://www.plantphysiol.org/cgi/content/full/135/3/1280/DC1.