Table I.
Phototropin mutants
| Type | Mutants | Antibiotics Resistancea | Number of Hybridized Fragmentsb |
|---|---|---|---|
| Single | photA1-1 | Km | 3 |
| photA2-1 | Km | 1 | |
| photA2-2 | Km | 6 | |
| photB1-1 | Km | 1 | |
| photB1-2 | Km | 1 | |
| photB2-1 | Km | 1 | |
| photB2-2 | Km | 3 | |
| Double | photA1photA2-1 | Km and Hyg | 2 |
| photA1photA2-2 | Km and Hyg | 2 | |
| photB1photB2-1 | Km and Hyg | 1 | |
| photB1photB2-2 | Km and Hyg | 1 | |
| Triple | photA2photB1photB2-1 | Km, Hyg, and Zeo | 1 |
| photA2photB1photB2-2 | Km, Hyg, and Zeo | 1 |
a
Km, kanamycin; Hyg, hygromycin; Zeo, Zeocin.
b
As hybridization probes, nptII, hpt, and Zeo genes were used for single, double, and triple mutants, respectively.