Figure 1.

Increase in [Ca²⁺]_cyt in response to H₂O₂ [Ca²⁺]_cyt was measured by luminometry as described in “Materials and Methods.” For each measurement, an individual 7-d-old reconstituted RLD1.1 seedling was placed into a plastic cuvette containing 0.5 mL water. H₂O₂ (final concentration 10 mm) was added. At 1,200 s, remaining aequorin was discharged. Traces represent the responses of three individual seedlings per treatment.