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. 2004 Jul;135(3):1565–1573. doi: 10.1104/pp.104.041699

Figure 3.

Figure 3.

Phenotype of the spds1-1 spds2-1 double mutant. A, Silique of a self-fertilized spds1-1/spds1-1 spds2-1/+ plant that was harvested 9 d after pollination. Arrows indicate spds1-1 spds2-1 double-mutant seeds that appeared brown and shrunken. Bar represents 0.5 μm. B and C, Nomarski images of a wild-type embryo (B) and an spds1-1 spds2-1 double-mutant embryo (C). Seeds were harvested from 3-d-old siliques after pollination. D and E, Light microscopy images of a wild-type embryo (D) and an spds1-1 spds2-1 double-mutant embryo (E) isolated from the mature dry seed. Bars represent 50 μm in B to E. F, Diagnostic PCR of the T-DNAs inserted in spds1-1 spds2-1. Genomic DNA was extracted from normal and arrested embryos. S1F and S1R, gene-specific primers for SPDS1; S2F and S2R, gene-specific primers for SPDS2; LB, primer specific to the T-DNA LB. Lanes 1, 3, 5, and 7, DNA from normal embryos; lanes 2, 4, 6, and 8, DNA from arrested embryos; lane M, DNA markers of indicated length.