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HPLC analysis of NCED inhibition activities. NCED activity was estimated in the presence of 23 μm 9′-cis-neoxanthin and 3.24 μg/mL enzyme at pH 7.0 at 20°C. The product, C25-apocarotenoid, was analyzed by HPLC at 440 nm wavelength. Error bars indicate the sd.
