Figure 4.

Northern-blot analyses of low-CO₂-inducible genes, LciA, LciB, and Mrp1. For detection of LciA and LciB mRNA, 30 μgs of total RNA isolated from wild-type (WT), ccm1, or pmp1-1 cells grown under high-CO₂ (H) or 1 h of low-CO₂ (L) were hybridized with gene specific ³²P-labeled probes. For detection of Mrp1 mRNA, 50 μgs of total RNA from cells grown in high-CO₂ (H) or 2 h of low-CO₂ (L) conditions. A ³²P-labeled cDNA probe for Cblp encoding the G-protein β-subunit, which is expressed constitutively, was used as a loading control.