Table IV.

Electrolyte leakage following freezing of supercooled (SC) nonacclimated and acclimated canola (cv Express) leaves

Nonacclimated Canola		Acclimated Canola
Duration of Freezing	Electrolyte Leakage	Duration of Freezing	Electrolyte Leakage
			−3°C		−6°C	−9°C	−12°C	−15°C
min	%	h	%
0	6.2	0.0	7.7	5.7	5.7	6.8
5	6.8	0.5	8.0	4.7	8.3	41
10	66	2.0	9.6	6.6	11	46
20	93	24	8.8	16	27	47
40	92

Canola leaves of similar size were excised from both nonacclimated and acclimated plants. In all cases the end of the excised petiole was coated with a silicone grease to prevent water loss and nucleation at the cut surface. Freezing of supercooled nonwetted leaves was initiated with ice crystals and the freezing events were monitored via IRVT. Nonacclimated canola leaves supercooled at −3°C for 5 to 40 min. Fully acclimated leaves were supercooled to −3°C, −6°C, −9°C, −12°C, or −15°C, then held isothermal for 0, 0.5, 2, or 24 h. Afterward, evaluation was conducted by electrolyte leakage.