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RNA gel-blot analysis of AtP5CS, ThP5CS, SOS1, and PDF1.2 genes in salt cress and Arabidopsis. Total RNA was prepared from 3-week-old Arabidopsis and 4-week-old salt cress plants that were the same in size during nonstress or 250 mm NaCl stress using the microarray analyses. Each lane was loaded with 10 μg of total RNA. The RNA was fractionated on a 1% agarose gel, blotted onto a nylon membrane, and hybridized with 32P-labeled AtP5CS, ThP5CS, SOS1, and PDF1.2 DNA fragments as probes.
