Table III.

Comparison of expression changes for selected salt cress transcripts

AGI	Function	Length	Log (C*S)	Log (S:C)	S:C MA	S:C PCR
At1g01720	ATAF1	77	3.727	0.632	4.28	4.72
At3g04120	GapC	68	5.353	0.542	3.48	2.43
At1g11910	Aspartic proteinase	94	4.426	0.020	1.05	1.22
At2g41430	ERD15	59	4.634	−0.552	0.28	0.24
At5g66190	Ferredoxin-NADP+ reductase	95	3.642	−0.118	0.76	0.65
At4g26080a	ABI1a	95	4.115	−0.259	0.55a	2.88a
At4g11650	Osmotin	70	4.503	−0.438	0.37	0.40
At3g44880	Lls1	94	3.887	0.154	1.43	0.99
At3g20410	CPK9	92	3.801	−0.420	0.38	0.52
At2g38540	LTP1	81	5.341	0.479	3.01	1.41

Compared are selected transcripts for which salt cress clones have been obtained by designing salt cress-specific primers and comparing their salt stress to control (S:C) ratio of expression changes determined by real-time RT-PCR (S:C-PCR) to those determined by microarray hybridization to the Arabidopsis long-oligo microarray platform (S:C-MA). Shown are the log₁₀ ratios for intensity (C*S), and log₁₀ (S:C) ratio from microarray experiments, and the fold-change in microarray (MA) and quantitative real-time RT-PCR (PCR) analysis.

^aThe microarray signal was obtained by hybridization to the printed Arabidopsis ABI1-specific oligonucleotide; the real-time PCR signal was obtained by using a primer pair for the cloned ABI1 homolog from salt cress (BQ079252). Several salt cress ESTs (gi|19684239; gi|19913611; gi|19855150; BQ07252), annotated as ABI1 homologs, seem to indicate the existence of ABI1 paralogs in salt cress that may be regulated in a different manner.