Table III.
Primers used for cloning of the wheat SPSIVa sequence by RT-PCR and 5′-RACE and for synthesis of wheat SPS and SPP probe
| Purpose | Primer Sequence (5′ → 3′) |
|---|---|
| Wheat SPSIVa Cloning | |
| RT-PCR1(F) | CMWGARTTTGTYGAYGGWGC |
| RT-PCR1(R) | CGRCAYTCWCCAAAIGCTTTNAC |
| RT-PCR2(F) | GGTCATRCTTGCAAGGAAGC |
| RT-PCR2(R) | GAAGYTTATASAGTGCATCTGC |
| 5′-RACE1 | GTGAACGTTAAGTGCTCCAG |
| 5′-RACE2 | GCAGCAACTCCTGCACTGCC |
| Hybridization Probes | |
| SPS I (F) | TACTGCGACCACCCGTCG |
| SPS I (R) | GTCATGCCGGTCGAGAGC |
| SPS II (F) | TACGCTCAAGTCTAGACATCC |
| SPS II (R) | CAAAGGATGTATCTCTGATGC |
| SPS III (F) | TTGACTCTTGGCATGAGATCTCC |
| SPS III (R) | CAAAACCAGCAGGATGCATGC |
| SPS IV (F) | TTAACTCTTAGCCCAAGATACC |
| SPS IV (R) | CTGTAGGAGCCATGCCTGC |
| SPS V (F) | GGAATTCCAGGACCTATCG |
| SPS V (R) | GACAATTGGGTGTCAGACCGGACCG |
| SPP 1 (F) | AACTTATGGTGAAGCCATGG |
| SPP 1 (R) | CTTCGCATTTTCTGCATGCC |
F, forward; R, reverse.