Table II.
Concentrations of amino acids (nmol [mg protein]−1) in tissue extracts
| Nodule | Frankia | Root | Sap | Leaf | |
|---|---|---|---|---|---|
| Ala | 40 ± 4 (3) | 61 ± 21 (8) | 11 (4) | 12 (12) | 101 (4) |
| Arg | 320 ± 101 (26) | 47 ± 12 (6) | 14 (5) | nd | 32 (1) |
| Asn | trace | nd | 12 (4) | nd | 11 (0) |
| Asp | 59 ± 9 (4) | 99 ± 28 (14) | 29 (10) | 21 (21) | 272 (10) |
| Cys | 5 ± 2 (1) | nd | 9 (3) | 0.6 (1) | 21 (1) |
| Gaba | 82 ± 16 (7) | nd | 31 (11) | 6.3 (7) | 266 (10) |
| Glu | 209 ± 31 (17) | 76 ± 2 (10) | 46 (17) | 28 (29) | 543 (19) |
| Gln | 401 ± 128 (33) | 45 ± 8 (6) | 81 (30) | 15 (16) | 1,160 (41) |
| Gly | 9 ± 2 (1) | 76 ± 24 (10) | 10 (4) | 3.8 (4) | 41 (1) |
| His | 19 ± 4 (2) | 17 ± 1 (2) | nd | nd | 14 (1) |
| Lys | 9 ± 2 (1) | 31 ± 4 (4) | nd | nd | 16 (1) |
| Orn | 1 ± 1 (0) | 48 ± 7 (7) | 5.4 (2) | 1.3 (1) | nd |
| Ser | 20 ± 2 (2) | 152 ± 29 (21) | 16 (6) | 4.6 (5) | 153 (5) |
| Thr | 21 ± 1 (2) | 51 ± 14 (7) | 11 (4) | 3.9 (4) | 63 (2) |
| Val | 8 ± 1 (1) | 25 ± 1 (3) | nd | nd | 41 (1) |
| Othera | ≤8 (≤1) | nd | nd | nd | ≤27 (≤1) |
Data for nodules are means ± se from two independent extracts; data for Frankia are means ± se of four determinations from three independent extracts; data for root, sap, and leaf are from single preparations. Numbers in parentheses represent percent of the total amino acid pool. “Sap” is fluid expressed from stem sections by centrifugation. Extracted protein (mg/mL): nodule 0.66 ± 0.09; Frankia 0.045 ± 0.017; root 0.25; sap 0.88; leaf 0.78. nd, none detected.
a
Ile, Leu, Met, Phe, Pro, Trp, Tyr.