Figure 3.

Localization of SLBP in cells treated with CHX or HU. HeLa cells expressing HA–SLBP were synchronized by double thymidine block. The cells were released into S phase for 3 h. Separate cultures were treated for 1 h with CHX (middle two panels) or HU (right two panels). Untreated cells in S phase are shown in the left two panels. Cells were fixed and the HA–SLBP detected by immunofluorescence (top panels) and DNA detected with DAPI (bottom panels) as described in Materials and Methods.