Table 1. Apparent dissociation constants for the interaction between mtCdc6/Orc1 proteins and short oligonucleotides.
| mthCdc6/Orc1 protein | DNA | Kd(app) (nM) |
|---|---|---|
| mthCdc6-1 wt | Single site | 230 ± 30 |
| mthCdc6-1 wt | Random site | 9200 ± 3400 |
| mthCdc6-1 S330A/S332A | Single site | 2300 ± 180 |
| mthCdc6-1 N341A/E342A | Single site | 350 ± 70 |
| mthCdc6-1 R334A/R335A | Single site | No binding detected |
| mthCdc6-1 R334A | Single site | No binding detected |
| mthCdc6-1 R335A | Single site | 960 ± 190 |
| mthCdc6-1 S355A | Single site | 210 ± 30 |
| mthCdc6-1 K360A | Single site | 530 ± 40 |
| mthCdc6-1 R362A | Single site | 540 ± 30 |
| mthCdc6-1 R358A | Single site | 510 ± 40 |
| mthCdc6-2 wt | Single site | 1400 ± 230 |
| mthCdc6-2 wt | Random site | No binding detected |