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Schematic map of the lead biosensor constructs; (a) pTOPOpbrRgfp and (b) pBBpbrRgfp. The pbrR gene (435 bp; blue), the divergent promoter region Ppbr (85 bp; pale blue) and a promoterless gfp gene (720 bp; green) were synthesized and cloned into pCR® 2.1-TOPO® TA (3931 bp). The lead biosensor genetic element (1240 bp) was then subcloned into the EcoRI site of the broad host range pBBR1MCS-5 (4768 bp) plasmid for transfer into other Gram-negative bacteria.
