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. 2004 Sep 8;32(16):e126. doi: 10.1093/nar/gnh119

Figure 3.

Figure 3

Figure 3

Assay sensitivity and linearity. (a) Electropherograms of 10-plex eTag mRNA assays run on ABI PRISM® 3100 Genetic Analyzer. CE marker 1 and 2 are the electrophoresis reference controls. An expanded view for low concentrations of target at 4 × 103 and 1 × 103 molecules per reaction is shown in the insert. (b) Average RPHs normalized to CE marker 2 in triplicates as a function of target concentration was plotted in double logarithm scale ranging from 1 × 103 to 6.25 × 107. (c) An expanded view for lower target concentration. A linear regression was performed on target concentration response curve for each gene. The LOQ and the coefficient of correlation for signal linearity curves are summarized in Table 1.