Table 1. The LOQ and coefficient of correlation of linearity assessed in a10-plex eTag multiplex mRNA assay.
Gene | Accession number | LOQa (molecules per reaction) | Coefficient of correlation (R2) |
---|---|---|---|
ICAM 1 | NM_000201 | 1 × 103 | 0.994 |
IL6 | M 14584 | 8 × 103 | 0.999 |
TNF | NM_000594 | 4 × 103 | 0.993 |
IL8 | NM_000584 | 4 × 103 | 0.988 |
CCL2 (MCP 1) | X14768 | 4 × 103 | 0.979 |
PTG S2 (COX 2) | NM_000963 | 4 × 103 | 0.995 |
ABCA 1 | NM_005502 | 4 × 103 | 0.995 |
SELE | NM_000450 | 4 × 103 | 0.999 |
VCAM 1 | NM_001078 | 1 × 103 | 0.997 |
GAPD | AK026525 | 4 × 103 | 0.999 |
aData from five replicates.