Table 2. Z′ factor calculated in 10-plex eTag mRNA assay.
| Gene | Normalized responses (to GAPD) | Z′ factor | |||||
|---|---|---|---|---|---|---|---|
| Positive control | Negative control | ||||||
| Mean (N = 12) | SD | CV% | Mean (N = 12) | SD | CV% | ||
| SELE | 5.07 | 0.36 | 7.10 | 0.08 | 0.01 | 12.5 | 0.72 |
| VCAM 1 | 1.82 | 0.19 | 10.61 | 0.08 | 0.01 | 12.5 | 0.69 |
| IL8 | 9.46 | 1.15 | 12.19 | 0.075 | 0.01 | 16.42 | 0.63 |
| COX 2 | 3.59 | 0.39 | 10.82 | 0.20 | 0.043 | 21.36 | 0.62 |
| IL6 | 1.38 | 0.17 | 12.36 | 0.08 | 0.011 | 15.06 | 0.58 |
| ICAM 1 | 5.10 | 0.70 | 13.72 | 0.08 | 0.012 | 16.40 | 0.57 |
| MCP 1 | 4.86 | 0.79 | 16.21 | 0.07 | 0.011 | 14.65 | 0.50 |
| ABCA 1 | 0.19 | 0.03 | 16.39 | 0.14 | 0.038 | 27.74 | NR |
SD, standard deviation; and NR, not reported due to statistically insignificant response.