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. 2004 Sep 8;32(16):e126. doi: 10.1093/nar/gnh119

Table 4. Comparison of gene expression change with real-time RT–PCR assay.

Gene eTag multiplex mRNA assay TaqMan® assay
  Fold change Normalized responses (N = 3) Fold changea CT number (N = 3)
    Untreated Treated   Untreated Treated
IL6 276.7 ± 89.3 0.002 ± 0.0003 0.55 ± 0.11 2436.8 ± 1184.5 34.0 ± 0.90 22.6 ± 0.10
IL8 70.40 ± 7.71 0.08 ± 0.01 5.63 ± 0.47 69.3 ± 6.30 24.4 ± 0.13 18.3 ± 0.04
ABCA1 1.32 ± 0.58 0.14 ± 0.04 0.19 ± 0.03 1.29 ± 0.15 27.0 ± 0.16 26.6 ± 0.29

aThe fold change measured in TaqMan® assay (47) was calculated as 2−ΔCt, where ΔCt = Ctuntreated − Cttreated. Calculation of Ct value was determined by interpolation of the RT–PCR progress curve.