Figure 8.

mtDNA nucleoid distribution and inheritance are disrupted in sporulating fission and dnm1/fusion mutants. (A) The distribution of Abf2-GFP-labeled mtDNA nucleoids was examined in wild-type and mutant strains during mitosis (black bars) and presporulation and sporulation/pretetrad stages (gray bars). Mitochondrial nucleoid appearance and distribution in single cells were scored as wild-type (five or more dispersed nucleoids or beads-on-a-string appearance) or abnormal (less than four nucleoids or aggregates). Abnormal Abf2-GFP nucleoid distribution is shown (n = total number scored). (B) Wild-type and mutant tetrad spores were scored for the presence or absence of Abf2-GFP-labeled mitochondrial nucleoids in each spore (n = total number scored).